解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Among 489 bats of 11 species in China, three novel paramyxoviruses [Tuhokovirus 1, 2 and 3 (ThkPV-1, ThkPV-2 and ThkPV-3)] were discovered in 15 Leschenault's rousettes. Phylogenetically, the three viruses are most closely related to Menangle and Tioman virus. Genome analysis showed that their 3'-leader sequences are ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.049
更新日期:2010-08-15 00:00:00
abstract::The V3 region of the HIV-1 envelope (Env) glycoprotein gp120 is a key functional domain yet it exhibits distinct mutational patterns across subtypes. Here an invariant residue (Ile 309) was replaced with Leu in 7 subtype C patient-derived Envs from recent infection and 4 related neutralizing antibody escape variants t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.04.010
更新日期:2010-08-15 00:00:00
abstract::The Crinivirus, Lettuce infectious yellows virus (LIYV) has a bipartite, positive-sense ssRNA genome. LIYV RNA 1 encodes replication-associated proteins while RNA 2 encodes proteins needed for other aspects of the LIYV life cycle. LIYV RNA 1 ORF 2 encodes P34, a trans enhancer for RNA 2 accumulation. Here we show that...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.04.006
更新日期:2010-07-20 00:00:00
abstract::Adeno-associated viruses (AAVs) are leading candidate vectors for human gene therapy. AAV serotypes have broad cellular tropism and use a variety of cellular receptors. AAV serotype 3 binds to heparan sulfate proteoglycan prior to cell entry and is serologically distinct from other serotypes. The capsid features that ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.027
更新日期:2010-07-20 00:00:00
abstract::Treatment with HIV attachment inhibitors (AIs) can select for escape mutants throughout the viral envelope. We report on three such mutations: F423Y (gp120 CD4 binding pocket) and I595F and K655E (gp41 ectodomain). Each displayed decreased sensitivity to the AI BMS-488043 and earlier generation AIs, along with increas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.033
更新日期:2010-07-05 00:00:00
abstract::We measured the effects of non-nucleoside reverse transcriptase (RT) inhibitor-resistant mutations K101E+G190S, on replication fitness and EFV-resistance of HIV(NL4-3). K101E+G190S reduced fitness in the absence of EFV and increased EFV resistance, compared to either single mutant. Unexpectedly, K101E+G190S also repli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.018
更新日期:2010-07-05 00:00:00
abstract::Mutations disrupting helicase domain motifs of the Tobacco mosaic virus 126/183-kDa proteins were investigated for their effect on replicase function and assembly. These mutations inhibited virus replication but did not affect 126-kDa induced N gene resistance or RNAi suppression. However, in vivo expressed 126-kDa mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.019
更新日期:2010-07-05 00:00:00
abstract::Powassan virus (POWV, Flaviviridae: Flavivirus) is the sole North American member of the tick-borne encephalitis complex and consists of two distinct lineages that are maintained in ecologically discrete enzootic transmission cycles. The underlying genetic mechanisms that lead to niche partitioning in arboviruses are ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.035
更新日期:2010-07-05 00:00:00
abstract::Hemagglutination is an established method but has not been used previously to determine the efficacy of virus binding to a specific cellular receptor. Here we have utilized CD46-expressing erythrocytes from a transgenic mouse to establish whether and to what extent the species B adenoviruses (Ads) as well as Ad37 and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.004
更新日期:2010-06-20 00:00:00
abstract::Activation of interferon regulatory factors (IRFs) 3 and 7 is essential for the induction of Type I interferons (IFN) and innate antiviral responses, and herpesviruses have evolved mechanisms to evade such responses. We previously reported that Epstein-Barr virus BZLF1, an immediate-early (IE) protein, inhibits the fu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.014
更新日期:2010-06-20 00:00:00
abstract::The 3' untranslated regions (UTRs) of many plant viral RNAs contain cap-independent translation elements (3' CITEs). Among the 3' CITEs, the Barley yellow dwarf virus (BYDV)-like translation elements (BTEs) form a structurally variable and widely distributed group. Viruses in three genera were known to harbor 3' BTEs,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.025
更新日期:2010-06-20 00:00:00
abstract::The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a hi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.036
更新日期:2010-05-25 00:00:00
abstract::Resistance to lethal encephalitis in mice infected with HSV-1 via the oral mucosa is mouse strain dependent. In susceptible BALB/c, HSV-1 spreads throughout the CNS but in resistant BL/6 mice, virus is restricted to the brainstem. To examine the contribution of cellular immunity in restricting viral spread, we used a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.02.013
更新日期:2010-05-10 00:00:00
abstract::Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear. Here, we show that >98% of HIV sequences contain consensus MAR element in their promoter. We show that SMAR1 binds to the LTR MAR and reinforces t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.017
更新日期:2010-04-25 00:00:00
abstract::While trying to generate a site-directed deletion in the ORF63 latency-associated gene of varicella-zoster virus (VZV) Oka, we constructed a virus with an unexpected rearrangement. The virus has a small deletion in both copies of ORF63 and two copies of a cassette inserted between ORFs 64/65 and 68/69 containing (a) t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.007
更新日期:2010-04-10 00:00:00
abstract::Marek' disease virus serotype-1, also know as Gallid herpesvirus 2 (GaHV-2), elicits T-cell lymphomas in chickens. The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that Meq homodimers are not sufficient to induce lymphomas in chickens. In this s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.006
更新日期:2010-04-10 00:00:00
abstract::Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.031
更新日期:2010-03-30 00:00:00
abstract::Previously different authors described various flavivirus mutants with high affinity to cell glycosaminoglycans and low neuroinvasiveness in mice that were obtained consequently passages in cell cultures or in ticks. In present study the analysis of TBEV isolates has shown existence of GAG-binding variants in natural ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.012
更新日期:2010-03-15 00:00:00
abstract::Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.005
更新日期:2010-03-15 00:00:00
abstract::Mammalian orthoreoviruses (reoviruses) are highly useful models for studies of double-stranded RNA virus replication and pathogenesis. We previously developed a strategy to recover prototype reovirus strain T3D from cloned cDNAs transfected into murine L929 fibroblast cells. Here, we report the development of a second...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.037
更新日期:2010-03-15 00:00:00
abstract::We previously demonstrated that Bombyx mori nucleopolyhedrovirus (BmNPV) multiplication is restricted in permissive BmN-4 cells upon coinfection with Hyphantria cunea NPV (HycuNPV). Here, we show that HycuNPV-encoded hycu-ep32 gene is responsible for the restricted BmNPV multiplication in HycuNPV-coinfected BmN-4 cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.049
更新日期:2010-03-15 00:00:00
abstract::In order to investigate the global and dynamic host microRNAs (miRNAs)/messenger RNAs (mRNAs) expression alteration during in vitro acute HCV infection, a comprehensive microarray analysis was performed using human hepatoma cells. Totally, 108 human miRNAs and 1247 mRNAs were identified whose expression levels changed...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.036
更新日期:2010-03-01 00:00:00
abstract::Human adenovirus (HAdV) is a cause of significant morbidity and mortality in immunocompromised patients, especially after stem cell transplantation (SCT). Viral clearance has been attributed to CD4(+) T-cell responses against the Hexon-protein, but the frequency of specific T(HELPER) cells is extremely low or not dete...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.049
更新日期:2010-02-20 00:00:00
abstract::In wild-type human parainfluenza virus type 2 (WT HPIV2), one gene (the P/V gene) encodes both the polymerase-associated phosphoprotein (P) and the accessory V protein. We generated a HPIV2 virus (rHPIV2-V(ko)) in which the P/V gene encodes only the P protein to examine the role of V in replication in vivo and as a po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.018
更新日期:2010-02-20 00:00:00
abstract::A central, yet unresolved issue in the pathogenesis of HIV disease is the mechanism of antibody perturbation. In this study, HIV-specific memory B-cells were quantified in groups of infected subjects and compared with memory responses to other antigens and antibody titers. HIV-specific memory B-cell responses were vig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.003
更新日期:2010-02-05 00:00:00
abstract::HIV-1 infection is characterized by life-long viral persistence and continued decline of helper CD4 T cells. The new generation of humanized mouse models that encompass RAG-hu, hNOG and BLT mice have been shown to be susceptible to HIV-1 infection and display CD4 T cell loss. Productive infection has been demonstrated...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.034
更新日期:2010-02-05 00:00:00
abstract::Here we address the role of RIG-I and TLR3 in differential cytokine responses against Simian Virus 5 (SV5) and two distinct cytokine inducing SV5 mutants. IFN-beta and IL-6 secretion was induced by infection with P/V-CPI-, an SV5 mutant with P/V substitutions, and were reduced by either siRNA-mediated knockdown of RIG...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.014
更新日期:2010-02-05 00:00:00
abstract::Human adenovirus type 3 (HAdV-B3) has an apparently stable genome yet remains a major circulating and problematic respiratory pathogen. Comparisons of the prototype genome to genomes from three current field strains, including two isolated from epidemics, and a laboratory strain, yielded small-scale nucleotide variati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.052
更新日期:2010-02-05 00:00:00
abstract::It is unresolved whether recently transmitted human immunodeficiency viruses (HIV) have genetic features that specifically favour their transmissibility. To identify potential "transmission signatures", we compared 20 full-length HIV-1 subtype C genomes from primary infections, with 66 sampled from ethnically and geog...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.002
更新日期:2010-01-20 00:00:00
abstract::Infection with a broad diversity of viruses often activates host cell signaling pathways including the mitogen-activated protein kinase pathway. The present study established that dengue virus infection of human macrophages activates Jun NH(2)-terminal kinase (JNK) and the p38 MAPKs pathways. The activation was observ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.019
更新日期:2010-01-05 00:00:00
abstract::The tail sheath protein of giant bacteriophage phiKZ Pseudomonas aeruginosa encoded by gene 29 was identified and its expression system was developed. Localization of the protein on the virion was confirmed by immunoelectron microscopy. Properties of gene product (gp) 29 were studied by electron microscopy, immunoblot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.09.015
更新日期:2009-12-20 00:00:00
abstract::The morbilliviruses are a closely related genus which are very similar in their sequences and share a common receptor, but nevertheless show significant restriction in the host species in which they cause disease. One contribution to this restriction might be the nature of the hosts' responses to infection. We have us...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.09.031
更新日期:2009-12-20 00:00:00
abstract::The Autographa californica M nucleopolyhedrovirus (AcMNPV) viral fibroblast growth factor (vFGF) has functional parallels to cellular FGFs. Deletion of the AcMNPV vfgf has no obvious phenotype in cell culture but delays the time of insect death. Here, we determined vFGF production during virus infection. vFGF was dete...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.09.011
更新日期:2009-12-05 00:00:00
abstract::Selection of high-growth virus variants of strain B/Victoria/504/2000 by serial passage in eggs resulted in three amino acid substitutions, G141E, R162M, and D196Y, in the vicinity of the receptor-binding pocket of viral hemagglutinin. Virus variants containing the identified amino acid substitutions, individually or ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.014
更新日期:2009-11-25 00:00:00
abstract::Human adenovirus type 5 (Ad5) has been the most popular platform for the development of oncolytic Ads. Alternative Ad serotypes with low seroprevalence might allow for improved anticancer efficacy in Ad5-immune patients. We studied the safety and efficacy of rare serotypes Ad6, Ad11 and Ad35. In vitro cytotoxicity of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.038
更新日期:2009-11-25 00:00:00
abstract:BACKGROUND:When antiretroviral therapy does not fully suppress HIV replication, suboptimal levels of antiretrovirals can select for antiretroviral resistant variants of HIV. These variants may exhibit reduced replication capacity and result in lower viral loads in blood. Our study evaluated whether antiretroviral resis...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.007
更新日期:2009-11-25 00:00:00
abstract::The extraordinarily high level of genetic variation of HIV-1 env genes poses a challenge to obtain antibodies that cross-react with multiple subtype Env glycoproteins. To determine if cross-reactive monoclonal antibodies (mAbs) to highly conserved epitopes in HIV-1 envelope glycoproteins can be induced, we immunized m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.041
更新日期:2009-11-10 00:00:00
abstract::Alpha human papillomaviruses (HPVs) are among the most common sexually transmitted agents of which a subset causes cervical neoplasia and cancer in humans. Alpha-PVs have also been identified in non-human primates although few studies have systematically characterized such types. We cloned and characterized 10 distinc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.012
更新日期:2009-10-25 00:00:00
abstract::To elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.023
更新日期:2009-10-25 00:00:00
abstract::Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.027
更新日期:2009-10-25 00:00:00